Detergents are active ingredients that humans use almost every day. This material has good washing powder so it is used to clean clothes. However, high detergent waste is known to pollute the environment so that it can disrupt the ecosystem in the environment. One alternative to replace detergent is natural detergent or what is called bio surfactant. These compounds are known to be produced by microorganisms. Based on these problems, in order to overcome environmental problems, one of the UM chemistry lecturers, Mieke Alvionita, and several chemistry students consisting of Nia Rahayu, Puspa Rahmadina, Chandra, and Bagas conducted a sampling to obtain bacteria that have the potential to produce bio surfactants. Sampling was carried out on July 10, 2021 at the Patuguran salt pond, Pasuruan Regency, East Java.

Sampling was carried out in several stages, namely (1) determination of the side point, (2) liquid and solid sampling, (3) measurement of pH and sample temperature, and (4) storage of samples in sterile bottles and in cooler boxes. The samples were then taken to the UM Biochemistry Research Laboratory. Dilution of the sample using sterile media which is then grown into a selective medium. This medium aims to select bacteria that have the most potential to produce bio surfactants. From this selection, there are 4 candidate bacteria that can produce bio surfactants. With this invention, it is hoped that the resulting bio surfactant can replace synthetic detergents for human welfare and health.



